Journal: Cancer Cell International

Article Title: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

doi: 10.1186/s12935-017-0446-2

Figure Lengend Snippet: Materials

Article Snippet: Anti-HIF-1α , Mouse monoclonal, clone 54, #610959, BD Transduction Laboratories™ , WB.

Techniques: Transduction

Journal: Cancer Cell International

Article Title: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

doi: 10.1186/s12935-017-0446-2

Figure Lengend Snippet: Recruitment of HIF-1α and ARNT to the ARNT gene promoter. a Functional elements within the ARNT promoter sequence upstream to −1200 bp from the transcription start site (bp 1). HBS HIF binding site, HAS HIF ancillary sequence, TATA TATA box. Investigated partial sequences are indicated (Region 1, Region 2, Region 3). b Chromatin immunoprecipitation (ChIP) assays. Values are presented as mean ± SEM of n = 3 independent experiments. IgG normal rabbit IgG, H3 histone H3

Article Snippet: Anti-HIF-1α , Mouse monoclonal, clone 54, #610959, BD Transduction Laboratories™ , WB.

Techniques: Functional Assay, Sequencing, Binding Assay, Chromatin Immunoprecipitation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep

doi: 10.3390/ani9090673

Figure Lengend Snippet: Immunostaining of four proteins in the heart of Tibetan sheep. All sections are from two-year-old sheep. ( A , B ): Hypoxia-inducible factor (HIF)-1α immunostaining in the heart; ( C , D ): HIF-2α immunostaining in the heart; ( E , F ): HIF-3α immunostaining in the heart; ( G , H ): Vascular endothelial growth factor (VEGF) immunostaining in the heart; ( I – L ): control of HIF-1α, HIF-2α, HIF-3α and VEGF in heart respectively. Arrows in ( B , D , H ): HIF-1α, HIF-2α and VEGF immunostaining in myocardial nuclei, respectively. The positive immunostaining showed brown with non-immunostained nuclei blue. ( A , C , E , G , I ): 20×; ( B , D , F , H , J – L ): 100×).

Article Snippet: Subsequently, they were incubated in a humid chamber overnight at 4 °C with a primary antibody of rabbit anti-HIF-1α, anti-HIF-2α, anti-HIF-3α or anti-VEGF polyclonal antibody (all antibodies diluted 1:200, Bioss, China, bs-20398R, bs-1447R, bs-5989R and bs-1665R), as described previously [ ].

Techniques: Immunostaining

Journal: Animals : an Open Access Journal from MDPI

Article Title: Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep

doi: 10.3390/ani9090673

Figure Lengend Snippet: Immunostaining of four proteins in the lung of two-year-old Tibetan sheep. ( A – C ): Hypoxia-inducible factor (HIF)-1α immunostaining in the lung; ( D – F ): HIF-2α immunostaining in the lung; ( G–I ): HIF-3α immunostaining in the lung; ( J–L ): Vascular endothelial growth factor (VEGF) immunostaining in the lung; ( M– P ): control of HIF-1α, HIF-2α, HIF-3α and VEGF in lung, respectively. Arrows in ( C , F , I , L ): HIF-1α, HIF-2α, HIF-3α and VEGF immunostaining in alveolar macrophages, respectively. The positive immunostaining was brown with non-immunostaining nuclei blue. ( A , D , G , J , M , O , P ): 20×; ( B , C , E , F , H , I , K , L , N ): 100×).

Article Snippet: Subsequently, they were incubated in a humid chamber overnight at 4 °C with a primary antibody of rabbit anti-HIF-1α, anti-HIF-2α, anti-HIF-3α or anti-VEGF polyclonal antibody (all antibodies diluted 1:200, Bioss, China, bs-20398R, bs-1447R, bs-5989R and bs-1665R), as described previously [ ].

Techniques: Immunostaining

Journal: Animals : an Open Access Journal from MDPI

Article Title: Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep

doi: 10.3390/ani9090673

Figure Lengend Snippet: The IOD (Integrated optical density) value of hearts and lungs of Tibetan sheep of different ages. VGEF is vascular endothelial growth factor, HIF is hypoxia-inducible factor. Values are means ± SD ( n = 10). * indicates that significant differences ( p < 0.05) in the IOD values existed between immunostaining intensity for VEGF and all three HIFs at that time point. + indicates significant differences in the immunostaining intensity of VEGF and HIF-1α in the heart and HIF-1α HIF-2α in the lung between 6-year-old and 1-year-old sheep.

Article Snippet: Subsequently, they were incubated in a humid chamber overnight at 4 °C with a primary antibody of rabbit anti-HIF-1α, anti-HIF-2α, anti-HIF-3α or anti-VEGF polyclonal antibody (all antibodies diluted 1:200, Bioss, China, bs-20398R, bs-1447R, bs-5989R and bs-1665R), as described previously [ ].

Techniques: Immunostaining

Journal: Animals : an Open Access Journal from MDPI

Article Title: Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep

doi: 10.3390/ani9090673

Figure Lengend Snippet: Hypoxia-inducible factor (HIF)-1α, HIF-2α, HIF-3α and vascular endothelial growth factor (VEGF) expression in the heart and lung of Tibetan sheep were measured by ELISA. Values are means ± SD ( n = 10). * indicates a significant difference in expression between 1-year-old and 6-year-old sheep ( p < 0.05).

Article Snippet: Subsequently, they were incubated in a humid chamber overnight at 4 °C with a primary antibody of rabbit anti-HIF-1α, anti-HIF-2α, anti-HIF-3α or anti-VEGF polyclonal antibody (all antibodies diluted 1:200, Bioss, China, bs-20398R, bs-1447R, bs-5989R and bs-1665R), as described previously [ ].

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep

doi: 10.3390/ani9090673

Figure Lengend Snippet: Hypoxia-inducible factor (HIF)-1α, HIF-2α, HIF-3α and vascular endothelial growth factor (VEGF) gene expression in the heart and lung of Tibetan sheep were measured by RTPCR (Reverse transcription polymerase chain reaction). Values are means ± SD ( n = 10). * indicates a significant difference ( p < 0.05) in gene expression within a tissue between 1-year-old and 6-year-old sheep. + indicates a significant difference ( p < 0.05) between gene expression in the heart and the expression of the same gene at the same age in the lung. The bar containing + has the significantly higher gene expression.

Article Snippet: Subsequently, they were incubated in a humid chamber overnight at 4 °C with a primary antibody of rabbit anti-HIF-1α, anti-HIF-2α, anti-HIF-3α or anti-VEGF polyclonal antibody (all antibodies diluted 1:200, Bioss, China, bs-20398R, bs-1447R, bs-5989R and bs-1665R), as described previously [ ].

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Foods

Article Title: Sea Buckthorn Pericarp Flavonoids Improve Diet-Induced Hyperlipidemia via Coordinated Modulation of Hepatic Lipid Metabolism and Gut Microbiota

doi: 10.3390/foods15061049

Figure Lengend Snippet: TFSP modulates the expression of ACC, FAS, CPT-1α, PPARα and ATGL proteins in the livers of HFD mice. n = 6 per group. Data are presented as mean ± SD. Different letters above bars indicate statistically significant differences ( p < 0.05) by one-way ANOVA followed by LSD post hoc test.

Article Snippet: Peroxisome proliferator-activated receptor alpha (PPARα) primary antibody (Cat. No. A00600-2), carnitine palmitoyltransferase-1 alpha (CPT-1α) primary antibody (Cat. No. A00917-3), acetyl-CoA carboxylase (ACC) primary antibody (Cat. No. M01802-2), fatty acid synthase (FAS) primary antibody (Cat. No. BA0484), adipose triglyceride lipase (ATGL) primary antibody (Cat. No. A01800-1), and GAPDH primary antibody (Cat. No. BM1623) were obtained from BOSTER Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing

Journal: Oncogene

Article Title: BATF2 prevents glioblastoma multiforme progression by inhibiting recruitment of myeloid-derived suppressor cells

doi: 10.1038/s41388-020-01627-y

Figure Lengend Snippet: A ELISA detection of MDSCs-associated cytokines in U251-Ctrl and U251-BATF2 tissues ( n = 3, independent experiment, * p < 0.05, ** p < 0.01, *** p < 0.001). B ELISA detection of SDF-1α in 7, 14, and 21 days in U251-Ctrl and U251-BATF2 tumour ( n = 3, independent experiment, * p < 0.05, ** p < 0.01, *** p < 0.001). C Representative IHC images of BATF2 and SDF-1α on consecutive sections of U251-Ctrl and U251-BATF2 intracranial tumours. Scale bars, 100 μm. D Representative images of immunofluorescent staining with SDF-1α (green), SV40 large T (red) and DAPI (blue) in U251-Ctrl and U251-BATF2 intracranial tumours. Scale bars, 25 μm. E Percentages of CXCR4 and CXCR7 expressing cells in CD11b + Gr1 + MDSCs isolated from tumours by FACs analysis. F Representative image of U251 tumour injected with U251-Ctrl- and U251-BATF2-derived EVs for four times, the subcutaneous tumour volume and SDF-1α detection by ELISA ( n = 3 independent experiment, * p < 0.05, ** p < 0.01, *** p < 0.001). G Western blot detection of HIF-1α and SDF-1α in 7, 14 and 21 days during U251 tumour progression. H EVs labelled by PKH67 added to phalloidin staining U251 cells and western blot detection of HIF-1α and SDF-1α. Scale bars, 20 μm. I ELISA and qPCR detection of SDF-1α in U251 tumour treated with Ctrl-EVs and BATF2-EVs ( n = 3, independent experiment, * p < 0.05, ** p < 0.01, *** p < 0.001). J ELISA detection of SDF-1α with and without 1% Triton X-100 under hypoxia condition ( n = 3, independent experiment, * p < 0.05, ** p < 0.01, *** p < 0.001). K Nano-FCM analysis and statistics of SDF-1α expression in Ctrl-EVs and BATF2-EVs ( n = 3, independent experiment, * p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Antibodies used in this study were as follows: BATF2 (ab157466, Abcam, London, UK); BATF2 (Thermo Fisher, PA5-43094); BATF2 (241887, Santa Cruz Biotechnology, CA, USA); CD63 (Abcam, ab217345); CD9 (Abcam, ab92726); TSG101 (Abcam, ab125011); SDF-1α (Abcam, ab9797); SDF-1α (Abcam, ab181018); HIF-1α (H6536, Sigma); CXCR4 (Abcam, ab135170); VEGF (ab46154, Abcam); CD33 (Abcam, ab203253); CD14 (Abcam, ab203294); Gr-1 (108448, Biolegend); CD11b (Abcam, ab13357); CXCR7 (Biolegend, #391405); SV40 Large T Antigen (CST, #15729, CA, USA); MMP-9 (CST, #13667); 10 nm gold-conjugated goat anti-rabbit secondary antibody (bs-0437P-Gold, Bioss, China), GAPDH (sc365062, Santa Cruz Biotechnology), and β-Actin (sc-47778, Santa Cruz Biotechnology).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Isolation, Injection, Derivative Assay, Western Blot

Journal: Oncogene

Article Title: BATF2 prevents glioblastoma multiforme progression by inhibiting recruitment of myeloid-derived suppressor cells

doi: 10.1038/s41388-020-01627-y

Figure Lengend Snippet: A Representative images of BATF2, CD33, CD14, SDF-1α, and HIF-1α in human malignant glioma tissues from 50 patients. Scale bars, 100 μm. B Correlations analyses of BATF2, CD33, CD14, SDF-1α, and HIF-1α in human malignant glioma tissues from 50 patients. The Pearson’s correlation coefficient ( r ) and p value are shown ( n = 50). C Exo-Counter detection of BATF2 + EVs in tumour tissues of I–II GBM, III–IV GBM and healthy donors ( n = 5, * p < 0.05, ** p < 0.01, *** p < 0.001). D Exo-Counter detection of BATF2 + EVs in plasma of I-II GBM, III-IV GBM and healthy donors ( n = 24, * p < 0.05, ** p < 0.01, *** p < 0.001). E ROC curve of BATF2 + EVs numbers data from I–II GBM vs. healthy donors, III–IV GBM vs. healthy donors, I–II GBM vs. III–IV GBM ( n = 24, * p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Antibodies used in this study were as follows: BATF2 (ab157466, Abcam, London, UK); BATF2 (Thermo Fisher, PA5-43094); BATF2 (241887, Santa Cruz Biotechnology, CA, USA); CD63 (Abcam, ab217345); CD9 (Abcam, ab92726); TSG101 (Abcam, ab125011); SDF-1α (Abcam, ab9797); SDF-1α (Abcam, ab181018); HIF-1α (H6536, Sigma); CXCR4 (Abcam, ab135170); VEGF (ab46154, Abcam); CD33 (Abcam, ab203253); CD14 (Abcam, ab203294); Gr-1 (108448, Biolegend); CD11b (Abcam, ab13357); CXCR7 (Biolegend, #391405); SV40 Large T Antigen (CST, #15729, CA, USA); MMP-9 (CST, #13667); 10 nm gold-conjugated goat anti-rabbit secondary antibody (bs-0437P-Gold, Bioss, China), GAPDH (sc365062, Santa Cruz Biotechnology), and β-Actin (sc-47778, Santa Cruz Biotechnology).

Techniques: